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单亲与双亲亲子鉴定结果的比较分析
于卫建, 梁晓华, 王旻, 孟庆丽
大连市红十字血液中心,辽宁 大连 116001
摘要:
[目的] 研究单亲和双亲亲子鉴定结果的准确性。[方法] 采用AmpFe STR TM Identifiler PlusTM荧光标记复合系统,通过310遗传分析仪对213例亲子鉴定进行基因型分型。[结果] 双亲排除案例的排除指标均为3个以上。单亲排除案例中,观察到3例排除指标小于3个,后经增加检测母亲遗传信息,排除指标均大于3个。排除案例中双亲组的排除指标比单亲组平均多2个。不排除案例中,双亲组的RCP均大于99.9999%;在单亲组中RCP值明显偏低,有29.3%案例低于99.73%,只有24%的案例RCP大于99.99%。[结论] 缺少一方的单亲鉴定对鉴定结果有明显影响。
关键词:  STR  亲子鉴定  亲子关系指数  基因鉴定
DOI:10.11724/jdmu.2005.02.30
分类号:R446.1
基金项目:
Analyze difference of paternity testing between single-parent group and double-parent goup
YU Wei-jian, LIANG Xiao-hua, WANG Min, MENG Qing-li
Dalian Red Cross Blood Center, Dalian 116001, China
Abstract:
[Objective] Study the accuracy of single-parent and double-parent paternity-testing.[Methods] AmpFe STR TM Identifiler Plus TM kit was used to perform PCR, the amplified products were processed through ABI PRISM310 genetic analyzer and the genotypes of all alleles was gotten through GeneScan software and GenoTyper software.[Results] In all excluding cases of double-parent group, the excluded STR loci were more than 3; compared in the simple-parent group that there were 3 cases the excluded STR loci were less than 3, when in those cases the mother sample was added, the excluded STR loci exceeded 3. The mean excluded STR loci in the double-parent group were 2 more than that in simple-parent group. In no-excluded cases, the RCP of all the double-parent group were more than 99.9999%, but in the simple-parent group, 29.3% cases were less than 99.73% and only 24% cases exceeded 99.99%. [Conclusion] Absence one of parent sample will obviously influence the paternity-testing result.
Key words:  STR  Paternity Testing  RCP  Gene-test
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