引用本文:
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 2551次   下载 1178 本文二维码信息
码上扫一扫!
分享到: 微信 更多
烟酰胺磷酸核糖转移酶在大鼠涎腺的表达及定位
赵西博1, 张 歆2, 李秀秀3, 高 媛3, 向 彬3, 张福胤1
1.大连医科大学 口腔医学院 口腔颌面外科,辽宁 大连 116044;2.大连育明高中,辽宁 大连 116023;3.大连大学 医学院 口腔系,辽宁 大连116622
摘要:
[目的]研究烟酰胺磷酸核糖转移酶(nicotinamide phosphoribosyl transferase,Nampt)在大鼠涎腺的表达和分布。[方法]应用半定量逆转录聚合酶链法(RT-PCR)和免疫印迹法检测大鼠正常腮腺、颌下腺和大舌下腺组织Nampt mRNA及蛋白质的表达。采用免疫组织化学法检测大鼠腮腺、颌下腺和大舌下腺组织Nampt的分布。[结果]大鼠腮腺、颌下腺和大舌下腺组织均有Nampt的mRNA及蛋白质表达,RT-PCR结果显示其表达相对光密度值分别为:腮腺82.04%,颌下腺50.89%,大舌下腺30.53%(与actin比较,P<0.05);Western Blot结果显示其表达相对光密度值分别为:腮腺77.34%,颌下腺47.61%,大舌下腺31.78%(与actin比较,P<0.05)。Nampt均主要分布于浆液性腺泡细胞的胞浆和胞核,以及闰管和纹状管的胞核中。[结论]大鼠腮腺、颌下腺和大舌下腺均存在Nampt的mRNA和蛋白质表达,为深入研究Nampt在涎腺生理及病理状态下的功能意义提供初步实验依据。
关键词:  烟酰胺磷酸核糖转移酶  腮腺  颌下腺  大舌下腺
DOI:10.11724/jdmu.2011.01.01
分类号:R780.2
基金项目:国家自然科学基金,辽宁省教育厅高校科研项目
Expression and localization of nicotinamide phosphoribosyl transferase in salivary glands of rat
ZHAO Xi-bo1, ZHANG Xin2, LI Xiu-xiu3, GAO Yuan3, XIANG Bin3, ZHANG Fu-yin1
1.Department of Oral Maxillofacial Surgery,School of Stomatology,Dalian Medical University,Dalian 116044,China;2.Dalian Yuming Senior High School,Dalian 116023,China;3.Department of Oral Medicine,Medical College,Dalian University,Dalian 116622,China
Abstract:
[Objective]To investigate the expression and distribution of nicotinamide phosphoribosyl transferase (Nampt) in rat salivary glands.[Methods]The expressions of Nampt mRNA and protein were investigated by reverse transcription-polymerase chain reaction (RT-PCR) and Western Blot in the parotid,submandibular and major sublingual glands of rat. Immunohistochemical assay was applied to detect the distribution of Nampt in these glands too.[Results]The results of RT-PCR showed that the optical density of the expression of Nampt mRNA was 82.04%,50.89% and 30.53% respectively in the parotid,submandibular and major sublingual glands of rat (P<0.05,compared with actin).The results of Western blot showed that the optical density of the expression of Nampt protein was 77.34%,47.61% and 31.78%,respectively in these glands (P<0.05,compared with actin).Nampt protein was widely distributed in them, mainly localized in the cytoplasm and nucleus of both serous acinar cells and ductal cells.[Conclusions]Nampt mRNA and protein are expressed in the parotid,submandibular and major sublingual glands of rat,and may be involved in underlying mechanisms of physiology and patho-physiology in the salivary glands.
Key words:  nampt  parotid gland  submandibular gland  major sublingual gland