引用本文:
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 次   下载 本文二维码信息
码上扫一扫!
分享到: 微信 更多
MOG抗原诱导的自身免疫性脑脊髓炎模型的建立及组织蛋白酶抑制剂cystatin F的表达
高文婷1, 范 凯2, 李 宁2, 马坚妹2
1.大连医科大学 实验动物中心,辽宁 大连 116044;2.大连医科大学 解剖学教研室,辽宁 大连 116044
摘要:
[目的]研究组织蛋白酶抑制剂cystatin F在少突胶质细胞糖蛋白(MOG)35-55诱导的自身免疫性脑脊髓炎(EAE)模型中的表达。[方法]利用MOG 35-55建立EAE模型,通过行为学观察对其进行神经功能评分;利用免疫组织化学方法证明髓鞘脱失情况;通过连续切片分析,及原位杂交和免疫组化技术对小胶质细胞活化和cystatin F的表达进行研究。[结果]利用MOG35-55可成功制备EAE模型;cystatin F在正常小鼠中枢神经系统内没有表达,但在脱髓斑块周围可见其表达。通过系列连续切片分析,其表达与在脱髓斑块周围积聚的小胶质细胞具有高度的一致性。[结论]cystatin F在MOG诱导的EAE中具有表达,其表达与积聚的小胶质细胞具有高度的一致性。
关键词:  自身免疫性脑脊髓炎模型  脱髓鞘  cystatin F  小胶质细胞
DOI:10.11724/jdmu.2011.01.02
分类号:R512.3
基金项目:国家自然科学基金,大连市优秀青年科技人才基金
Establishment of experimental autoimmune encephalomyelitis model and the expression of cystatin F
GAO Wen-ting1, FAN Kai2, LI Ning2, MA Jian-mei2
1.Experimental Animal Center;2.Department of Anatomy,Dalian Medical University,Dalian 116044,China
Abstract:
[Objective]To study the expression of cystatin F,which is cathepsin protease inhibitor,in experimental autoimmune encephalomyelitis model induced by myelin oligodendrocyte glycoprotein 35-55.[Methods]Myelin oligodendrocyte glycoprotein 35-55 was used to establish experimental autoimmune encephalomyelitis model in C57BL/6 mice,then clinical score was estimated by behaviou observation.Immunohistochemistry method was used to understand demylinating conditon in model mice,and in situ hybridyzation method was performed to know the activity of microglia cells and the expression of cystatin F.[Results]Experimental autoimmune encephalomyelitis model induced by myelin oligodendrocyte glycoprotein 35-55 was successful.Accumulated activated microglia cells were found around the demyelinating plaque,at meantime cystatin F expression were also found.[Conclusion]The expression of cystatin F in demyelinating disease was correlated with activated microglia cells.
Key words:  experimental autoimmune encephalomyelitis  demyelination  cystatin F  microglia cells