| Objective To establish of obstructive colon cancer model in BALb/c mice with method and verify its stability. Methods The 50 BALb/c mice were randomized into two groups, 25 obstructive colon cancer model group (study group) and 25 PBS replaces cancer cells model group (control group). After the model, the mice were divided into five sub-groups, and executed in postoperative 3, 7, 10, 14 and 21 day respectively. BALb/c mice of general conditions, simulative cancerous intestinal obstruction of surveilance method, and histopathological examination were evaluated in two groups. Results The rates of tumor formation was 100.0% (25/25) in the study group. BALb/c mice model of general conditions of data analysis, study group had a significantly losses mean food intake, weight, and excrement in the postoperative 10 days (P<0.05). The simulative cancerous intestinal obstruction of surveilance method of data analysis, study group showed significantly mean increase obdominal circumference, intestinal pressure, and circumference of the intestinal which located at upper the obstruction site in the postoperative 7 days (P<0.05). The histopathological examination of data analysis, tumor formation time was postoperative 7 days, and colon circumferential growth and obstructive colon cancer of times were postoperative 14 days. Conclusion The modified method to establish a BALb/c mice of obstructive colon cancer model are operational simple, reduces intestinal injury, technically feasible, oncological safety, loss mortality, and higher stability. That has basically reflected pathological physiological evolution of clinical obstructive colon cancer, and are desired animal models.