金樱子胶囊对四氯化碳诱导小鼠急性肝损伤的保护作用研究
Protective effect of Rosa laevigata Michx capsule against liver injury  in mice induced by carbon tetrachloride
投稿时间:2018-01-15  
DOI:10.11724/jdmu.2018.01.05
中文关键词:  金樱子胶囊  四氯化碳  肝损伤  氧化应激
英文关键词:Rosa laevigata Michx capsule  CCl4  liver injury  oxidative stress
基金项目:
作者单位
尹连红 大连医科大学 药学院辽宁 大连 116044 
许有威 大连医科大学 药学院辽宁 大连 116044 
摘要点击次数: 63
全文下载次数: 81
中文摘要:
      目的 研究金樱子胶囊对四氯化碳(CCl4)诱导小鼠急性肝损伤的保护作用。方法 昆明小鼠随机分为空白对照组、模型组和金樱子胶囊不同剂量(100 mg/kg、200 mg/kg、400 mg/kg,ig)给药组;给药7 d后,以腹腔注射0.4% CCl4花生油溶液(0.1 mL/10 g)的方法建立急性肝损伤模型,HE染色观察小鼠肝脏组织病理学损伤,试剂盒检测小鼠血清谷草转氨酶(AST)、谷丙转氨酶(ALT)及肝组织超氧化物歧化酶(T-SOD)、丙二醛(MDA)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-PX)含量。结果 金樱子胶囊能显著改善肝脏组织损伤,逆转CCl4诱导的小鼠肝中SOD、GSH-PX、GSH活力下降,降低MDA含量,并显著降低血清中AST和ALT的含量。结论 金樱子胶囊可显著改善小鼠肝中氧化应激水平,对CCl4诱导的小鼠急性肝损伤具有一定的保护作用。
英文摘要:
      ObjectiveTo observe the protective effect of Rosa laevigata Michx capsule against liver injury in mice induced by carbon tetrachloride (CCl4).MethodsMice were randomly divided into the control group, the model group and different doses of Rosa laevigata Michx health food group[100 mg/(kg·d), 200 mg/(kg·d), 400 mg/(kg·d)]. After administration 7 d, we established the model of liver injury via intraperitoneal injection of 0.4% (CCl4) (0.1 mL/10 g), andthen observe the pathology changes. Furthermore, we detected AST and ALT levels in serum, and MDA, SOD, GSH and GSH-PX levels in hepatic tissue.ResultsRosa laevigata Michx capsule could notably improved the injury of liver tissue and significantly increased liver SOD, GSH-PX and GSH activities and remarkably decreased MDA, AST and ALT in mice after treated by CCl4.ConclusionsRosa laevigata Michx capsule has a protective effect against liver injury in mice induced by CCl4 through regulating oxidative stress levels.
查看全文  查看/发表评论  下载PDF阅读器