| Objective Retrospective analysis of clinical data of 71 cases of poisonous mushroom poisoning, to evaluate the clinical efficacy of hemodialysis (HD), hemoperfusion (HP), plasma exchange (PE) therapies for mushroom poisoning and to provide more theoretical basis for optimizing clinical treatment of poisonous mushroom poisoning. Methods According to different treatment methods, the patients were divided into 4 groups including routine therapy group (19 cases), HD group (12 cases), HP group (23 cases), and HP combined with PE group (17 cases). The HD group patients were dynamically monitored for Scr, Urea and urine volume. The other three groups of patients showed varying degrees of liver, coagulation and nervous system damage. No kidney damage was observed. These patients were monitored for serum level changes of AST, ALT, TB, PT-T, and APTT after admission to the hospital. Results After treatment, the HD group patients had progressively decreased Scr and Urea levels and gradually increased urine volume (P<0.05). After seven days of treatment, serum levels of AST, ALT and TB decreased significantly in HP combined with PE group compared to conventional treatment group and HP group; serum levels of AST and ALT declined more in HP group than those in conventional treatment group. HP combined PE treatment corrected the patients’ PT-T and APTT markedly faster than HP (P<0.05). Conclusion Hemodialysis should be used as soon as possible to treat acute injury caused by poisonous mushroom poisoning. The application of hemoperfusion combined with plasma exchange improves liver function and coagulation function and can obtain satisfactory curative effect in the short term.