| Objective To compare the clinical features of invasive breast cancer with different Ki67 expression and to explore the relationship between Ki67 expression and various prognostic parameters. Methods Totally 315 patients with invasive breast cancer, who underwent surgery in our hospital, were enrolled. According to the Ki67 expression level, they were divided into low expression group (Ki67≤14%) and high expression group (Ki67>14%). The prognostic parameters of the two groups were recorded and summarized, including tumor size, lymph node metastasis, histological grade, ER expression, PR expression, HER-2 expression and molecular subtype. Results There was no significant difference in lymph node metastasis between the two groups (P>0.05). There were significant differences on tumor size, histological grade, ER expression, PR expression, HER-2 expression and molecular subtype (P<0.05). Conclusion The clinical features of invasive breast cancer with different Ki67 expression are different. Ki67 can be used in invasive breast cancer as an indicator to obtain prognostic information and may help for therapeutic decisions.